解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The leader RNA transcript of vesicular stomatitis virus (VSV) has been immunoprecipitated from infected BHK cell extracts by anti-La specific sera from patients with systemic lupus erythematosus (SLE). This association was specific as lupus anti-sera with other specificities failed to precipitate leader RNA. The amoun...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90541-x
更新日期:1983-10-01 00:00:00
abstract::We have isolated cDNA clones generated from the mRNA encoding the opsin apoprotein of bovine rhodopsin and used these cDNAs to isolate genomic DNA clones containing the complete opsin gene. Nucleotide sequence analysis of the cloned DNAs has yielded a complete amino acid sequence for bovine rhodopsin and provided an i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90537-8
更新日期:1983-10-01 00:00:00
abstract::An antiserum against material shed into serum-free medium by MCF-7 human mammary carcinoma cells (anti-SFM II) disrupts cell-cell interactions in murine mammary tumor epithelial cells (MMTE). We now report purification of an 80 kd glycoprotein (GP80) from SFM of MCF-7 mammary carcinoma cells that blocks the activity o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90379-3
更新日期:1983-09-01 00:00:00
abstract::We have analyzed the structure of the Shrunken (Sh) locus in a strain containing an unstable recessive mutation, sh-m5933, caused by the transposable controlling element Dissociation (Ds). We have also analyzed nine spontaneous Sh revertant alleles. The sh-m5933 allele contains a 30 kb insertion at the Sh locus, as we...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90372-0
更新日期:1983-09-01 00:00:00
abstract::The role of various segments (gag or v-abl) of the Abelson murine leukemia virus (A-MuLV) genome in both lymphoid cell and fibroblast transformation was examined by deletion of areas from cloned, plasmid DNA representations of the genome. The deleted plasmids were tested by transfection into fibroblasts and by infecti...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90389-6
更新日期:1983-09-01 00:00:00
abstract::Mutant strains of D. discoideum are described that can complete morphogenesis and cytodifferentiation but which express vastly reduced levels of the galactose-binding lectins discoidin I and II (less than 1% and 1%-2% respectively) compared to the wild-type control. Mutant cells proceeding through development lack lec...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90380-x
更新日期:1983-09-01 00:00:00
abstract::The transport kinetics of the influenza virus hemagglutinin from its site of synthesis to the apical plasma membrane of Madin-Darby canine kidney cells, a polarized epithelial cell line, were studied by a sensitive tryptic assay. Hemagglutinin acquired terminal sugars, as judged by sensitivity to endo-beta-N-acetylglu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90154-x
更新日期:1983-08-01 00:00:00
abstract::We have isolated and characterized several members of the P transposable element family from a Drosophila melanogaster P strain. Large 2.9 kb elements are present as multiple highly conserved copies together with smaller (0.5-1.6 kb), heterogeneous elements. The complete DNA sequences of the 2.9 kb element and four sm...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90133-2
更新日期:1983-08-01 00:00:00
abstract::The murine major histocompatibility complex I region encodes two class II antigens, I-A and I-E. From a mouse spleen DNA cosmid library of the b haplotype, we isolated a clone containing the entire I-A beta gene and a separate exon encoding a beta-chain second domain (A beta 2). The A beta gene, encompassing more than...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90148-4
更新日期:1983-08-01 00:00:00
abstract::Recessive lethal mutations in the T/t-complex of the mouse characteristically show defective genetic complementation, even when they affect very different stages of embryogenesis and are known to be nonallelic. To address the question of their genetic or functional relationship, we have applied the cis-trans test, usi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90035-1
更新日期:1983-07-01 00:00:00
abstract::At early times after adenovirus infection, transcription from all early viral promoters requires the expression of a 289 amino acid phosphoprotein encoded in the pre-early transcription unit E1A. Mutations in E1A result in a marked delay in the induction of early viral transcription. To distinguish between possible mo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90011-9
更新日期:1983-07-01 00:00:00
abstract::We have examined the molecular basis of the response of individuals of D. melanogaster to artificial selection for high and low abdominal bristles. By monitoring the fate of particular rDNA spacer length variants associated with individually isolated X and Y chromosomes, we show that flies from the low bristle number ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90027-2
更新日期:1983-07-01 00:00:00
abstract::We have tested a model for global ribosome biosynthesis by examining the effects of increased gene dosage on the synthesis of rRNA. Increasing gene dosage does not lead to a significant increase in total rRNA transcription; i.e., rRNA synthesis from individual rRNA operons is reduced to keep total rRNA production unch...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90029-6
更新日期:1983-07-01 00:00:00
abstract::We investigated soybean lectin gene structure and expression in a genetic line that accumulates normal levels of seed lectin (Le+ line) and in a mutant line that produces no detectable seed lectin (Le- line). We found that the nuclear and cytoplasmic lectin RNA levels in Le- embryos are approximately 0.01% of those in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90428-2
更新日期:1983-06-01 00:00:00
abstract::We have shown that the DNA sequences adjacent to the telomeres of Saccharomyces cerevisiae chromosomes are highly conserved and contain a high density of replication origins. The salient features of these telomeres can be summarized as follows. There are three moderately repetitive elements present at the telomeres: t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90437-3
更新日期:1983-06-01 00:00:00
abstract::Primary cell cultures of African Green monkey kidney (AGMK) contain polarized epithelial cells in which influenza virus matures predominantly at the apical surfaces above tight junctions. Influenza virus glycoproteins were found to be localized at the same membrane domain from which the virus budded. When polarized pr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90425-7
更新日期:1983-06-01 00:00:00
abstract::Mutations in four nuclear genes, kar1 cdc4, 28, and 37, block or impair nuclear fusion during conjugation of Saccharomyces cerevisiae. Mutations in all four genes are recessive for the caryogamy defect; in matings between diploid cells both of which are heterozygous for any one of the four mutations (-/+ X -/+), caryo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90349-5
更新日期:1983-05-01 00:00:00
abstract::When treated with the synthetic glucocorticoid dexamethasone, HT1080 human fibrosarcoma cells show changes in morphology, adhesion, and the extracellular matrix. Dexamethasone treatment results in a tenfold increase in the rate of fibronectin biosynthesis in HT1080 cells and a twofold increase in untransformed, normal...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90357-4
更新日期:1983-05-01 00:00:00
abstract::We show that expression of the iso-1-cytochrome c gene of Saccharomyces cerevisiae, CYC1, is tightly regulated by levels of intracellular heme. Expression is reduced at least 200-fold in cells grown under conditions of heme deficiency. Studies on the regulation of a CYC1-lacZ fused gene and direct determination of mRN...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90309-4
更新日期:1983-04-01 00:00:00
abstract::We have used monoclonal antibodies to detect developmentally regulated cell surface antigens on D. discoideum amebae. A study of an antigen detected using an antibody produced by a hybridoma line implicates a previously undescribed component in the process of cell aggregation. This antigen (consisting of a doublet of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90295-7
更新日期:1983-04-01 00:00:00
abstract::A mutant in src, the oncogene of Rous sarcoma virus, has been constructed in which the major phosphorylated tyrosine (Tyr-416, located in the carboxy-terminal half of the protein) has been replaced by phenylalanine. Mouse cells transformed with this mutant src form foci and grow in soft agar, indicative of a transform...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90074-0
更新日期:1983-03-01 00:00:00
abstract::We determined the nucleotide sequences of all coding regions and a significant part of the flanking regions of the chicken c-src gene, which is a cellular homolog of the v-src gene of Rous sarcoma virus. The c-src gene consists of 12 exons; the boundaries of the exons were determined by assuming that the amino acid se...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90073-9
更新日期:1983-03-01 00:00:00
abstract::The eye lens contains a structural protein (alpha-crystallin), composed of two homologous primary gene products, alpha A2 and alpha B2. In certain rodents, there is another minor alpha-crystallin polypeptide, alpha Ains, which is identical to alpha A2 except for a 22 amino acid insert between residues 63 and 64 of the...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90056-9
更新日期:1983-03-01 00:00:00
abstract::Recombinant E. coli that produce light were found in a clone library of hybrid plasmids containing DNA from the marine bacterium Vibrio fischeri. All luminescent clones had a 16 kb insert that encoded enzymatic activities for the light reaction as well as regulatory functions necessary for expression of the luminescen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90063-6
更新日期:1983-03-01 00:00:00
abstract::Salmonella possesses several flagella, each capable of counterclockwise and clockwise rotation. Counterclockwise rotation produces swimming, clockwise rotation produces tumbling. Switching between senses occurs stochastically. The rotational sense of individual flagella on a single cell could be monitored under specia...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90501-9
更新日期:1983-01-01 00:00:00
abstract::Replication of Escherichia coli plasmid p15A was examined by use of a cell extract or a mixture of three purified E. coli enzymes: RNA polymerase; RNAase H; and DNA polymerase I. In each system, replication initiates at any of three consecutive nucleotides located at a unique site. Primer transcription starts 508 bp u...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90502-0
更新日期:1983-01-01 00:00:00
abstract::We have isolated spontaneous temperature-resistant revertants of a temperature-sensitive mutation (rpoD800) in the sigma subunit of E. coli K12 RNA polymerase. These revertants still contained the rpoD800 allele. They were mucoid, and sensitive to ultraviolet light and the radiomimetic agent nitrofurantoin, which are ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90505-6
更新日期:1983-01-01 00:00:00
abstract::A diverse range of ultimate chemical carcinogens inhibited the transfer of methyl groups from S-adenosylmethionine to hemimethylated DNA in a reaction catalyzed by mouse spleen methyltransferase. The formation of alkali-labile sites in DNA lessened its ability to accept methyl groups in vitro, but the methylation reac...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90514-7
更新日期:1983-01-01 00:00:00
abstract::We describe a monoclonal antibody (anti-FC-1) that inhibits the attachment of chicken embryo fibroblasts to artificial substrates. In the immunofluorescence, anti-FC-1 also stained the focal contacts of the fibroblasts, organelles suggested to be involved in cell-substrate adhesion. This antibody was absorbed on the s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90322-1
更新日期:1982-12-01 00:00:00
abstract::The susceptibility to repair of particular base mismatches by the hex system of Streptococcus pneumoniae was examined by comparison of the nucleotide sequence of the wild-type and eight mutant alleles of the malM gene. A detailed restriction map was constructed for pLS70, and the nucleotide sequence was determined for...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90126-x
更新日期:1982-12-01 00:00:00
abstract::The pluripotential hemopoietic stem cells (CFU-S) of genetically resistant Fv2rr adult C57BL/6 (B6) mice can be persistently infected by Friend virus (FV) when exposed to defective spleen focus-forming virus (SFFV) and helper virus (LLV) at the time of transplantation into heavily irradiated syngeneic recipients (that...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90327-0
更新日期:1982-12-01 00:00:00
abstract::Transcriptional analysis of SV40 late promoter mutants indicates that the DNA sequence 5'-GGTACCTAACC-3' (map positions 294-304) is important in the control of SV40 late RNA expression. C to T base substitutions at map positions 298, 299 and 304 increased initiation of late RNA synthesis at map position 325 five to te...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90318-x
更新日期:1982-12-01 00:00:00
abstract::Homology is an important feature of recombination. We have used the rll cistrons of bacteriophage T4 to determine the extent of homology required for recombination. We varied the amount of homologous DNA available for recombination in both marker rescue experiments and deletion-by-deletion crosses. Our results suggest...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90401-9
更新日期:1982-11-01 00:00:00
abstract::The linear extrachromosomal ribosomal DNA of Tetrahymena is generated from a single integrated copy during macronuclear development. The free ends of this extrachromosomal gene contain 20-70 tandem repeats of the hexanucleotide CCCCAA. We have determined the nucleotide sequence at the same (3') end of the single, inte...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90417-2
更新日期:1982-11-01 00:00:00
abstract::We have fused DNA fragments derived from an Escherichia coli tRNALeu1 operon to the galK gene of E. coli to identify sequences necessary for the in vivo initiation of transcription and growth-rate-dependent regulation. Promoter sequences consisting of residues from -50 to +56 or -50 to +5 with respect to the in vivo s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90290-2
更新日期:1982-10-01 00:00:00
abstract::We have prepared three monoclonal antibodies against human epidermal keratins. These antibodies were highly specific for keratins and, in combination, recognized all major epidermal keratins of several mammalian species. We have used these antibodies to study the tissue distribution of epidermis-related keratins. In v...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90234-3
更新日期:1982-09-01 00:00:00
abstract::Methylation of deoxycytidine incorporated by DNA excision-repair was studied in human diploid fibroblasts following damage with ultraviolet radiation, N-methyl-N-nitrosourea, or N-acetoxy-2-acetylaminofluorene. In confluent, nondividing cells, methylation in repair patches induced by all three agents is slow and incom...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90248-3
更新日期:1982-09-01 00:00:00
abstract::A plasmid, pHR402, containing SV40 sequences that include a truncated early region bearing an intact t-coding sequence and a functionally intact late region, was introduced into thymidine kinase deficient (tk-) mouse L cells by cotransformation with a cloned tk gene. tk+ cotransformants synthesized SV40 t but not T an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90244-6
更新日期:1982-09-01 00:00:00
abstract::Periodic transcription of yeast histone genes has been demonstrated by DNA excess filter hybridization of in vivo pulse-labeled RNA isolated from synchronous cell cultures. Using strains carrying cell division cycle (cdc) mutations, we show that both activation and termination of transcription are determined by tempor...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90036-8
更新日期:1982-08-01 00:00:00
abstract::We have employed repeat units of herpes simplex virus (HSV) defective genomes to derive a cloning-amplifying vector (amplicon) that can replicate in eucaryotic cells in the presence of standard HSV helper virus. The design of the HSV amplicon system is based on the previous observation that cotransfection of cells wit...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90035-6
更新日期:1982-08-01 00:00:00